DETECTION OF ETHANOL IN A 2-COMPONENT GLUCOSE ETHANOL MIXTURE USING ANONSELECTIVE MICROBIAL SENSOR AND A GLUCOSE ENZYME ELECTRODE/

Chemometric theory was applied to a microbial sensor for determination s of ethanol in the presence of glucose. Microbial sensors, consisting of Gluconobacter oxydans cells immobilized on Clark-type amperometric oxygen electrodes, exhibited good sensitivity but low selectivity tow ard ethanol and glucose. An Eksan-G commercial glucose analyzer was us ed as a second sensor for multivariate calibration and analyses. Micro bial sensors exhibited nearly complete additivity for total glucose pl us ethanol concentrations from 0.0 to 0.6 mM. Within this linear range , chemometric analyses provided estimates of ethanol concentration wit h measurement errors of less than 8%. Multivariate calibration thus is a promising approach to enhance the usefulness of microbial sensors. (C) 1998 Elsevier Science S.A. All rights reserved.