VOLTAMMETRIC BEHAVIOR OF MITOXANTRONE AT A DNA-BIOSENSOR

The surface of an electrochemical glassy carbon electrode was modified with a layer of double-stranded DNA (dsDNA) or with double-stranded D NA conditioned in single-stranded DNA (ssDNA) and was used to investig ate mitoxantrone-DNA interactions. Differential pulse and square wave voltammetry were applied to develop an electroanalytical procedure for the determination of mitoxantrone and evaluate its interaction with d sDNA or ssDNA immobilized on the electrode surface. The results demons trate that MTX interaction with DNA is not specific to either guanine or adenine bases. The kinetics of the mitoxantrone-DNA interaction is slow and damage to DNA was followed with time. (C) 1998 Elsevier Scien ce S.A. All rights reserved.