AMPEROMETRIC BIOSENSORS BASED ON SOLID BINDING MATRICES APPLIED IN FOOD QUALITY MONITORING

Solid binding matrix (SBM) based composite transducers have been used for development of series of multibiosensor systems applicable in vari ous fields. Here we present two hybrid three-channel;multibiosensors f or simultaneous amperometric operation in food quality control, i.e. g lucose/fructose/ethanol multibiosensor, based on glucose oxidase/fruct ose dehydrogenase/alcohol dehydrogenase surface-modified enzyme electr odes and L-lactate/L-malate/sulfite multibiosensor, based on L-lactate dehydrogenase/L-malate dehydrogenase/sulfite oxidase surface-modified enzyme electrodes. Different parameters have been studied in order to optimize the response of the multibiosensor systems. The multibiosens or showed a good sensitivity, linear range and storage stability. The multibiosensors were used for the determination of glucose, fructose, ethanol, L-lactate, L-malate and sulfite in samples of wine, resulting in a good agreement with data certified by the supplier. Comparison o f various designs, surface-modified, bulk-modified and thick-cover, of SBM based biosensors is studied on the example of fructose biosensor. (C) 1998 Elsevier Science S.A. All rights reserved.