SUPPRESSION OF ELECTROPHORETIC ANOMALY OF BENT DNA SEGMENTS BY THE STRUCTURAL PROPERTY THAT CAUSES RAPID MIGRATION

Citation
T. Ohyama et al., SUPPRESSION OF ELECTROPHORETIC ANOMALY OF BENT DNA SEGMENTS BY THE STRUCTURAL PROPERTY THAT CAUSES RAPID MIGRATION, Nucleic acids research, 26(21), 1998, pp. 4811-4817
Citations number
36
Categorie Soggetti
Biology
Journal title
ISSN journal
03051048
Volume
26
Issue
21
Year of publication
1998
Pages
4811 - 4817
Database
ISI
SICI code
0305-1048(1998)26:21<4811:SOEAOB>2.0.ZU;2-9
Abstract
Intrinsic DNA curvature is speculated to be a common feature of all sa tellite DNA sequences and may aid in the tight winding of DNA in const itutive heterochromatin. Several satellite DNAs, however, show unusual ly rapid migration in non-denaturing polyacrylamide gels, which is jus t the opposite behavior of that shown by curved DNA structures. Employ ing bovine satellite I DNA monomer, we attempted to understand the mol ecular mechanism of 'rapid migration'. The phenomenon of rapid migrati on was temperature-dependent and to a small extent polyacrylamide-conc entration-dependent. Physiological or near-physiological concentration s of Mg2+ and Ca2+ ions bent the rapid migrating DNA segment, Predomin ance of purine-purine base stacking over purine-pyrimidine in nucleoti de sequence was strongly indicated to be the cause of the rapid migrat ion, Furthermore, they seemed to be implicated in the formation of ind uced DNA bend. We also found that the satellite I monomer contains an intrinsic DNA curvature as do many other satellites, Heretofore, the r apid migration property has concealed the presence of curvature.