ELECTRON-TRANSFER BETWEEN AN ELECTROCHEMICALLY DEPOSITED GLUCOSE-OXIDASE CU[II] COMPLEX AND AN ELECTRODE

Glucose oxidase (GOD) complexed with Cu[II] was deposited on the elect rode surface with retention of enzyme activity by an electrochemical r eduction of Cu[II]. The deposited GOD showed an apparently reversible electron transfer to an electrode through the coordinated Cu. The GOD/ Cu electrode responded to glucose in anodic current due to an electroc hemical oxidation of enzymatically generated FADH. In conclusion, the electron transfer was mediated by bound Cu which was situated at an ac tive center of GOD. The GOD/Cu system provided an ''atomic interface'' to perform a smooth electron transfer between a flavoenzyme and an el ectrode. (C) 1998 Elsevier Science S.A. All rights reserved.