INACTIVATION OF MONOAMINE-OXIDASE-B BY OMETHYL-3-(4-METHOXYPHENYL)DIHYDROFURAN-2(3H)-ONES AND OMETHYL-3-(4-METHOXYPHENYL)DIHYDROFURAN-2(3H)-ONES

Monoamine oxidase B was previously shown to be inactivated by cis- (3) and omethyl)-3-(4-methoxyphenyl)dihydrofuran-2(3H)-one hydrochloride (4) in a time-dependent manner (Ding, Z.; Silverman, R. B. J. Med. Che m. 1992, 35, 885) and to catalyze its oxidative decarboxylation (Silve rman, R. B.; Zhou, J. J. P.; Ding, C. Z.; Lu, X. J. Am. Chem. Sec. 199 5, 117, 12895). By [C-14]-labeling of the aryl methoxyl groups of thes e two inactivators, it is shown that this is not a mechanism-based ina ctivation and that multiple enzyme residues are labeled. (C) 1998 Else vier Science Ltd. All rights reserved.