GBV-C HGV COINFECTION IN HIV-1-POSITIVE MEN - FREQUENT DETECTION OF VIRAL-RNA IN BLOOD-PLASMA BUT ABSENCE FROM SEMINAL FLUID PLASMA/

Sequential paired samples of blood and seminal fluid were obtained fro m a cohort of 54 HIV-1-infected homosexual males. The prevalence of GB V-C/HGV RNA in the cell-free fractions of some of these patients was d etermined using reverse-transcription polymerase chain reaction (RT-PC R). To assess the effects of HIV-1 and HCV infection upon GBV-C/HGV RN A status, blood CD4 cell counts, HCV RNA status, and HIV-1 proviral DN A and viral RNA titres were also determined. GBV-C/HGV RNA was detecte d in 8/30 (27%) of the blood plasma samples obtained at the start of t he study, and was present at a frequency of 14/64 (22%) in all the blo od plasma samples tested. By contrast, GBV-C/HGV RNA was not detected in the 26 seminal fluid samples obtained at the start of the study, in cluding 8 samples obtained from patients for which GBV-C/HGV RNA was d etected in the corresponding blood sample. Of the samples tested for t he presence of both GBV-C/HGV and HCV RNA, there was no evidence of co infection. Although GBV-C/HGV RNA detection rates were significantly h igher in individuals with blood CD4 cell counts greater than 200 cells per microlitre, there were no significant differences in the median b lood CD4 cell counts or HIV-1 proviral DNA or viral RNA titres observe d between the GBV-C/HGV-positive and -negative individuals. The failur e to detect GBV-C/HGV RNA in seminal fluid samples obtained from this cohort would suggest that further studies need to be carried out to de termine the roles of sexual transmission and of seminal fluid in GBV-C /HGV infection. (C) 1998 Wiley-Liss, Inc.