NEUTROPHILS SECRETING TUMOR-NECROSIS-FACTOR-ALPHA INFILTRATE THE CENTRAL-NERVOUS-SYSTEM OF BALB C MICE WITH EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS/

Experimental autoimmune encephalomyelitis (EAE) can be induced in resi stant BALB/c mice by ultrasound-formed adjuvant emulsion. In contrast to susceptible mouse strains large numbers of neutrophils secreting TN F-ol occupied the central nervous system (CNS) of BALB/c mice with sev ere EAE, whereas only small numbers of macrophages and CD4(+) T-cells could be detected. CNS infiltration was preceded with activation of mi croglial cells. Ultrasound formed adjuvant induced early IFN-gamma exp ression in popliteal lymph nodes of BALB/c mice, whereas conventional adjuvant induced delayed lFN-gamma production. Although the clinical o utcome of EAE was similar to that seen in susceptible mice, the pathog enesis was distinct having possible implications on the different form s seen in multiple sclerosis. (C) 1998 Elsevier Science B.V. An rights reserved.