USE OF A LIPOSOME ANTIGEN DELIVERY SYSTEM TO ALTER IMMUNE-RESPONSES IN-VIVO

It has been reported that a certain peptide encompassing residues 129- 140 of the hepatitis B virus core antigen (HBcAg) leads to a Th2-type response in C57BL/10 mice. We postulated that by formulating the pepti de in liposomes along with an immune modulator known as MPLA the immun e response could be directed toward a Th1-type response. If these lipo somes could deliver the peptide along with MPLA to antigen presenting cells, then the immune response generated could be polarized to a Th1 response. The type of immune response initiated after immunization wit h the peptide HBcAg (126-140) in different formulations was determined by an ex vivo T cell proliferation assay and by analysis of the cytok ine profile of the proliferating T cells. A group of C57BL/6 mice immu nized with peptide plus MPLA in a liposome formulation displayed a str ong T cell proliferative response. The T cell subset was identified as Th1 based on the cytokine profile. The cytokine profiles showed signi ficant production of interferon-gamma (IFN-gamma, a Th1-type cytokine) and extremely low levels of interleukin-4 (IL-4, a Th2-type cytokine) . The control group of C57BL/6 mice immunized with peptide plus alum s howed a very low level of T cell proliferation, and no increase was se en in IFN-gamma or IL-4 production. These data signify that a Th1-type response occurred in mice treated with peptide in a liposome formulat ion but not in mice treated with the control formulation.