ESTABLISHMENT OF THE ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR CONNECTIVE-TISSUE GROWTH-FACTOR (CTGF) AND ITS DETECTION IN THE SERA OF BILIARY ATRESIA

Authors
TAMATANI T KOBAYASHI H TEZUKA K SAKAMOTO S SUZUKI K NAKANISHI T TAKIGAWA M MIYANO T
Citation
T. Tamatani et al., ESTABLISHMENT OF THE ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR CONNECTIVE-TISSUE GROWTH-FACTOR (CTGF) AND ITS DETECTION IN THE SERA OF BILIARY ATRESIA, Biochemical and biophysical research communications (Print), 251(3), 1998, pp. 748-752
Citations number
23
Categorie Soggetti
Biology,Biophysics
Journal title
Biochemical and biophysical research communications (Print) → ACNP
ISSN journal
0006291X
Volume
251
Issue
3
Year of publication
1998
Pages
748 - 752
Database
ISI
SICI code
0006-291X(1998)251:3<748:EOTEFC>2.0.ZU;2-#
Abstract
Connective tissue growth factor (CTGF) is a mitogenic, chemotactic, an d cell matrix-inducing factor for fibroblasts. We generated murine mon oclonal antibodies against CTGF and established a sandwich enzyme-link ed immunosorbent assay (ELISA) for detection of CTGF. By using the ELI SA, we confirmed that CTGF was specifically induced in human fibroblas ts by TGF-beta but not by PDGF, FGF, IGF-I, or EGF. We also found that the serum levels of CTGF were significantly correlated with the progr ession of hepatic fibrosis in biliary atresia. These results indicated that CTGF is potentially a useful parameter for monitoring certain ty pes of fibrotic disorders. (C) 1998 Academic Press.