PROMOTER ANALYSIS OF THE ACETATE-INDUCIBLE ISOCITRATE LYASE GENE (ACU-3) FROM NEUROSPORA-CRASSA

Analysis of the promoter region of the acetate-induced isocitrate lyas e gene (acu-3) of Neurospora crassa was undertaken. A series of deleti ons in the 5' non-transcribed region were constructed and the effects of these mutations on the enzyme levels following growth on sucrose an d transfer to acetate were measured. Sequences within the region -603 to -271 relative to the transcription start site appear essential for transcription. The region -950 to -1278 is required for sucrose repres sion, which is consistent with previous protein/DNA gel retardation re sults of protein extracts from N. crassa cultured on sucrose. Protein extracts from acetate-induced mycelia identify alternative promoter re gions apparently involved in acetate-induced gene transcription. (C) 1 998 Elsevier Science B.V. All rights reserved.