M. Bibbins et al., PROMOTER ANALYSIS OF THE ACETATE-INDUCIBLE ISOCITRATE LYASE GENE (ACU-3) FROM NEUROSPORA-CRASSA, Biochimica et biophysica acta, N. Gene structure and expression, 1442(2-3), 1998, pp. 320-325
Analysis of the promoter region of the acetate-induced isocitrate lyas
e gene (acu-3) of Neurospora crassa was undertaken. A series of deleti
ons in the 5' non-transcribed region were constructed and the effects
of these mutations on the enzyme levels following growth on sucrose an
d transfer to acetate were measured. Sequences within the region -603
to -271 relative to the transcription start site appear essential for
transcription. The region -950 to -1278 is required for sucrose repres
sion, which is consistent with previous protein/DNA gel retardation re
sults of protein extracts from N. crassa cultured on sucrose. Protein
extracts from acetate-induced mycelia identify alternative promoter re
gions apparently involved in acetate-induced gene transcription. (C) 1
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