A NOVEL VARIANT OF TRANSLATION ELONGATION FACTOR-1-BETA - ISOLATION AND CHARACTERIZATION OF THE RICE GENE ENCODING EF-1-BETA-2

Authors
TERUI Y TSUTSUMI K KIDOU S SAWAZAKI T KUROIWA Y YAMAKI M EJIRI S
Citation
Y. Terui et al., A NOVEL VARIANT OF TRANSLATION ELONGATION FACTOR-1-BETA - ISOLATION AND CHARACTERIZATION OF THE RICE GENE ENCODING EF-1-BETA-2, Biochimica et biophysica acta, N. Gene structure and expression, 1442(2-3), 1998, pp. 369-372
Citations number
16
Categorie Soggetti
Biology,Biophysics
Journal title
Biochimica et biophysica acta, N. Gene structure and expression → ACNP
ISSN journal
01674781
Volume
1442
Issue
2-3
Year of publication
1998
Pages
369 - 372
Database
ISI
SICI code
0167-4781(1998)1442:2-3<369:ANVOTE>2.0.ZU;2-V
Abstract
A rice gene encoding a novel isoform of translation elongation factor- 1 beta subunit (termed EF-1 beta 2) was isolated and characterized. Th e gene comprises of eight exons, and encodes a 226-amino-acid protein. Expression of EF-1 beta 2 mRNA is abundant in seeds and cultured cell s, but is considerably low in the tissues of the rice seedling. Antise rum raised against an EF-1 beta 2 synthetic peptide detected a protein with a relative molecular mass of about 32 kDa, indicating the EF-1 b eta 2 gene is actually expressed in rice tissues. EF-1 beta 2 showed a close similarity to the cognate subunits from plant (beta and beta'). (C) 1998 Elsevier Science B.V. All rights reserved.