DETECTION OF POLYMORPHIC FGF RECEPTOR-1 MESSENGER-RNA IN BOVINE LENS EPITHELIUM BY PCR

Authors
MARUNOUCHI T HOSOYA H KOUSAKA M LEE XR MAJIMA Y
Citation
T. Marunouchi et al., DETECTION OF POLYMORPHIC FGF RECEPTOR-1 MESSENGER-RNA IN BOVINE LENS EPITHELIUM BY PCR, Biochemistry and molecular biology international, 41(6), 1997, pp. 1171-1178
Citations number
12
Categorie Soggetti
Biology
Journal title
Biochemistry and molecular biology international → ACNP
ISSN journal
10399712
Volume
41
Issue
6
Year of publication
1997
Pages
1171 - 1178
Database
ISI
SICI code
1039-9712(1997)41:6<1171:DOPFRM>2.0.ZU;2-U
Abstract
The expression of FGFR 1, 2 and 3 but not FGFR 4 was detected in bovin e lens epithelial cells. FGFR 1 expression of both the 2-Ig and 3-Ig f orms was detected by a difference in the lengths of the PCR products. Furthermore, mRNA which included or excluded six nucleotides correspon ding to two amino acids in the acid box region of FGFR 1 was detected by a difference in mobility of the homo- and hetero-duplex strands for med in the PCR reaction.