THE AFM AS A TOOL FOR CHROMOSOMAL DISSECTION - THE INFLUENCE OF PHYSICAL PARAMETERS

Human metaphase chromosomes were dissected using an atomic force micro scope (AFM) in ambient conditions and in buffer. Cutting with z-modula tion in air yielded precise cuts at loading forces F > 17 mu N with a full width at maximum depth of 90 nm. After dissection, the chromosoma l material adhered to the tip to be used for further biochemical proce ssing. In liquids, we measured the effects of different types of buffe r solution on swelling of the chromosomes and their elastic behaviour.