THE ESCHERICHIA-COLI CMLA GENE ENCODES THE MULTIDRUG EFFLUX PUMP CMR MDFA AND IS RESPONSIBLE FOR ISOPROPYL-BETA-D-THIOGALACTOPYRANOSIDE EXCLUSION AND SPECTINOMYCIN SENSITIVITY/

Authors
BOHN C BOULOC P
Citation
C. Bohn et P. Bouloc, THE ESCHERICHIA-COLI CMLA GENE ENCODES THE MULTIDRUG EFFLUX PUMP CMR MDFA AND IS RESPONSIBLE FOR ISOPROPYL-BETA-D-THIOGALACTOPYRANOSIDE EXCLUSION AND SPECTINOMYCIN SENSITIVITY/, Journal of bacteriology (Print), 180(22), 1998, pp. 6072-6075
Citations number
30
Categorie Soggetti
Microbiology
Journal title
Journal of bacteriology (Print) → ACNP
ISSN journal
00219193
Volume
180
Issue
22
Year of publication
1998
Pages
6072 - 6075
Database
ISI
SICI code
0021-9193(1998)180:22<6072:TECGET>2.0.ZU;2-8
Abstract
Expression of cloned genes from isopropyl-beta-D-thiogalactopyranoside (IPTG)-regulated promoters is lowered when the Escherichia coli CmlA/ Cmr/MdfA efflux pump is is overexpressed, probably due to IPTG exclusi on from the cytoplasm. The previously reported cmlA1 mutation confers a similar phenotype, cmlA1 contains an IS30 insertion upstream of cmr/ mdfA, which creates a putative promoter. CmlA overproduction also caus es spectinomycin hypersensitivity.