CONSTRUCTION AND PRIMARY ANALYSIS OF HUMAN KERATINOCYTE MODEL EXPRESSING ANTISENSE PROTEIN-KINASE C-ZETA

Protein kinase G (PKC), which has a wide variety of functions, compris es at least twelve isoenzymes that have distinct roles respectively. R esearch in recent years indicates that PKC zeta plays a pivotal role i n the regulation of cell proliferation. In order to investigate its ro le in the regulation of keratinocyte proliferation, eukaryotic express ion plasmid of anti-sense PKC zeta is constructed and transfected into human keratinocyte line, Colo16. The human keratinocyte model express ing anti-sense PKC zeta is established successfully, the growth proper ties of the cell model is analyzed by the detection of cell growth cur ve and cell cycle phase. The mechanism of the relationship between PKC zeta and CyclinD1 is also investigated by the western blotting analys is of CyclinD1 protein.