CONSTITUENTS OF HOLOTHUROIDEA - 7 - ISOLATION AND STRUCTURE OF BIOLOGICALLY-ACTIVE GANGLIOSIDES FROM THE SEA-CUCUMBER HOLOTHURIA-PERVICAX

Three ganglioside molecular species, HPG-8, HPG-3, and HPG-1, have bee n obtained from the polar fraction of the chloroform/methanol extract of the sea cucumber Holothuria pervicax. The structures of these gangl iosides have been determined on the basis of chemical and spectroscopi c evidence. They represent new ganglioside molecular species. HPG-8 is a sulfated monosialo-ganglioside, while HPG-3 and HPG-1 are disialo-g angliosides possessing 2-->4-linked tandem-type disialosyl moieties. T hese three gangliosides qualitative neuritogenic activity toward the r at pheochromocytoma cell Line. PC-12 cells.