DETERMINATION OF INTERNUCLEAR ANGLES OF DNA USING PARAMAGNETIC ASSISTED MAGNETIC ALIGNMENT

Paramagnetic ions have been used to assist the magnetic alignment of D NA. The anisotropy of the binding sites is sufficient to give rise to significant alignment of the DNA with the observed proton-carbon dipol ar couplings spanning a 70-Hz range. The dipolar couplings have been u sed to determine the positions of the axial and rhombic alignment axes . The positions of the alignment axes relative to the positions of the binding sites of the paramagnetic europium ions have also been determ ined. (C) 1998 Academic Press.