Mcg. Monao et al., DETECTION OF JC VIRUS-DNA IN HUMAN TONSIL TISSUE - EVIDENCE FOR SITE OF INITIAL VIRAL-INFECTION, Journal of virology (Print), 72(12), 1998, pp. 9918-9923
Progressive multifocal leukoencephalopathy is a demyelinating disease
of the human central nervous system that results from lytic infection
of oligodendrocytes by the polyomavirus JC (JCV). Originally, JCV was
thought to replicate exclusively in human glial cells, specifically ol
igodendrocytes. However, we have recently shown that JCV can replicate
in cells of lymphoid origin such as hematopoietic precursor cells, B
lymphocytes, and tonsillar stromal cells. To determine whether tonsils
harbor JCV, we tested a total of 54 tonsils, 38 from children and 16
from adult donors. Nested PCRs with primer sets specific for the viral
T protein and regulatory regions were used for the detection of JCV D
NA. JCV DNA was detected in 21 of 54 tonsil tissues, or 39% (15 of 38
children and 6 of 16 adults) by using regulatory-region primers and in
19 of 54 tonsil tissues, or 35% (13 of 38 children and 6 of 16 adults
) by using the T-protein primers. The DNA extracted from children's no
ndissected tonsil tissue, isolated tonsillar lymphocytes; and isolated
stromal cells that demonstrated PCR amplification of the JCV regulato
ry region underwent cloning and nucleotide sequencing. Of the regulato
ry-region sequences obtained, nearly all contained tandem repeat arran
gements. Clones originating from non-dissected tonsil tissue and tonsi
llar lymphocytes were found to have sequences predominantly of the Mad
-1 Prototype strain, whereas the majority of clones from the DNA of to
nsillar stromal cells had sequences characteristic of the Mad-8(br) st
rain of JCV. A few clones demonstrated structures other than tandem re
peats but were isolated only from tonsillar lymphocytes. These data pr
ovide the first evidence of the JCV genome in tonsil tissue and sugges
t that tonsils may serve as an initial site of viral infection.