ALKOXYPSORALENS, NOVEL NONPEPTIDE BLOCKERS OF SHAKER-TYPE K- SYNTHESIS AND PHOTOREACTIVITY( CHANNELS )

A series of psoralens and structurally related 5,7-disubstituted couma rins was synthesized and investigated for their K+ channel blocking ac tivity as well as for their phototoxicity to Artemia salina and their ability to generate singlet oxygen and to photomodify DNA. After scree ning the compounds on Ranvier nodes of the toad Xenopus Laevis, the af finities of the most promising compounds, which proved to be psoralens bearing alkoxy substituents in the 5-position or alkoxymethyl substit uents in the neighboring 4- or 4'-position, to a number of homomeric K + channels were characterized. All compounds exhibited the highest aff inity to Kv1.2. 5,8-Diethoxypsoralen (10d) was found to be an equally potent inhibitor of Kv1.2 and Kv1.3, while lacking the phototoxicity n ormally inherent in psoralens. The reported compounds represent a nove l series of nonpeptide blockers of Shaker-type K+ channels that could be further developed into selective inhibitors of Kv1.2 or Kv1.3.