ELECTRONEGATIVE LOW-DENSITY-LIPOPROTEIN SUBFORM (LDL-) IS INCREASED IN TYPE-2 (NON-INSULIN-DEPENDENT) MICROALBUMINURIC DIABETIC-PATIENTS AND IS CLOSELY ASSOCIATED WITH LDL SUSCEPTIBILITY TO OXIDATION
E. Moro et al., ELECTRONEGATIVE LOW-DENSITY-LIPOPROTEIN SUBFORM (LDL-) IS INCREASED IN TYPE-2 (NON-INSULIN-DEPENDENT) MICROALBUMINURIC DIABETIC-PATIENTS AND IS CLOSELY ASSOCIATED WITH LDL SUSCEPTIBILITY TO OXIDATION, Acta diabetologica, 35(3), 1998, pp. 161-164
There is increasing evidence that diabetes mellitus is characterized b
y an enhanced lipoprotein oxidation. We have therefore investigated wh
ether a relationship exists between LDL oxidation and microalbuminuria
, which is considered an early marker of vascular involvement in type
2 diabetic patients. We selected 12 microalbuminuric and 12 normoalbum
inuric type 2 diabetic patients, and 12 control subjects comparable fo
r age, sex and blood pressure sure values. Oxidatively modified plasma
LDL, referred as LDL-, were measured by ion-exchange HPLC. In vitro s
usceptibility to oxidation of LDL was evaluated by following the kinet
ics of conjugated diene formation in the presence of Cu++ ions (lag-ph
ase time). Microalbuminuric diabetic patients had a less satisfactory
metabolic control and showed a higher plasma triglyceride concentratio
n than both normoalbuminuric diabetic patients (2.21 +/- 1.01 vs 1.15
+/- 0.39 mmol/l, P < 0.01) and controls (1.18 +/- 0.61 mmol/l, P < 0.0
1). The percentage of LDL- in plasma was significantly increased in mi
croalbuminuric diabetic patients in comparison with both normoalbuminu
ric diabetic patients (5.24 +/- 1.67 vs 3.13 +/- 1.22%, P < 0.01) and
controls (2.34 +/- 1.03%, P < 0.001). LDL isolated from microalbuminur
ic diabetic patients had a significantly shorter lag-phase time in com
parison with normoalbuminuric diabetic patients (79 +/- 11 vs 97 +/- 1
0 min, P < 0.05) and controls (120 +/- 24 min, P < 0.001). In diabetic
patients a significant linear correlation was observed between the pe
rcentage of LDL- and amount of fructosamine (r = 0.45, P < 0.05), HbA(
1c) (r = 0.41, P < 0.05), and triglycerides (r = 0.65, P < 0.001). An
inverse correlation was found between lag-phase time and fructosamine
(r = -0.5, P < 0.01) and triglycerides (r = -0.59, P < 0.001). This st
udy shows that microalbuminuric type 2 diabetic patients had evidence
of increased LDL oxidation, which seems to be mainly due to a poor met
abolic control and a more atherogenic lipid profile.