P. Kuronen et al., REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SCREENING METHOD FOR SERUM STEROIDS USING RETENTION INDEX AND DIODE-ARRAY DETECTION, Journal of chromatography B. Biomedical sciences and applications, 718(2), 1998, pp. 211-224
Citations number
62
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical sciences and applications
A multisteroid screening method has been developed based on the use of
1-[4-(2,3-dihydroxypropoxy)phenyl]-1-alkanones as retention index sta
ndards and UV absorbance spectra recorded on-line with a diode-array d
etector using reversed-phase high-performance liquid chromatographic g
radient elution with acetonitrile and water. The effect of chromatogra
phic conditions on retention indices of steroids were studied. The met
hod was tentatively applied to profiling of steroids in serum samples.
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