A. Juszkiewicz et al., CALORIMETRIC STUDY OF INHIBITION OF UREASE BY 2-MERCAPTOETHANOL - PROCEDURES BASED UPON INTEGRATED RATE-EQUATIONS, Thermochimica acta, 320(1-2), 1998, pp. 45-52
Urease-catalyzed hydrolysis of urea was studied in the absence, and pr
esence, of 0.3 and 0.8 mmol dm(-3) 2-mercaptoethanol in phosphate buff
er at pH 7.0 at 25 degrees C with the use of an isoperibol calorimeter
. The extent of reaction with time, Delta T vs. t, was interpreted wit
h the help of the integrated Michaelis-Menten equation, and the inhibi
tion constant K-i was obtained from linear transformations of the equa
tion (Jennings-Niemann, Yun-Suelter and Booman-Niemann). The obtained
value of K-i was equal to 0.87+/-0.10 mmol dm(-3). (C) 1998 Elsevier S
cience B.V.