DETERMINATION OF S R RATIO OF MEPHENYTOIN IN HUMAN URINE BY CHIRAL HPLC AND ULTRAVIOLET DETECTION AND ITS COMPARISON WITH GAS-CHROMATOGRAPHY/

Citation
Sl. Huang et al., DETERMINATION OF S R RATIO OF MEPHENYTOIN IN HUMAN URINE BY CHIRAL HPLC AND ULTRAVIOLET DETECTION AND ITS COMPARISON WITH GAS-CHROMATOGRAPHY/, Zhongguo yaoli xuebao, 19(6), 1998, pp. 548-550
Citations number
10
Categorie Soggetti
Pharmacology & Pharmacy",Chemistry
Journal title
ISSN journal
02539756
Volume
19
Issue
6
Year of publication
1998
Pages
548 - 550
Database
ISI
SICI code
0253-9756(1998)19:6<548:DOSRRO>2.0.ZU;2-0
Abstract
AIM: To improve HPLC method for rapid determination of urinary S/R-rat io of mephenytoin, a widely used metabolic index for cytochrome P-450 2C19 (CYP2C19) activity. METHODS: Aliquots of 0 - 8-h urine sample aft er dosing racemic mephenytoin 100 mg underwent one-step extraction wit h dichloromethane. Analysis was performed on a chiral column (250 mm x 4 mm, 5 mu m) at lambda = 207 nm. The eluent was a mixture of acetron itrile and water containing both 0.1% glacial acetic acid and 0.2% tri ethylamine (14:86, vol/vol) at a now-rate of 0.9 mL . min(-1). RESULTS : The enatiomers of mephenytoin in urine were well separated within 9 min.; A linear correlation was observed between 50 - 5000 mu g . L-1 w ith the detection limit of 12.5 mu g . L-1 for both enantiomers of mep henytoin. This HPLC analysis was comparable to gas chromatography in a ccuracy and sensitivity, but with much shorter retention time and bett er resolution. CONCLUSION: The present HPLC method is good for rapid d etermination of the ability of subjects to hydroxylate S-mephenytoin a fter oral administration of the racemic drug.