Enzymatic hydrolysis of starch has traditionally been performed in lar
ge volume batch reactors using soluble enzyme during long time. Introd
uction of the membrane bioreactor brings possibility of continuous ope
ration in lower volume reactors in shorter reaction time. Studies on t
he simultaneous enzyme action of glucoamylase and pullulanase on lique
fied potato starch in a continuous membrane reactor were made with the
application of the various cut-off and transmembrane pressure of memb
ranes. Influence or different reaction conditions namely enzyme concen
tration, substrate: enzyme ratio and residence time on glucose content
were measured. Membrane reactor with 3,000 Da produced almost pure gl
ucose at resilience time of 3-4 h.