THE EFFECT OF RECOMBINANT HUMAN GRANULOCYTE MACROPHAGE-COLONY-STIMULATING FACTOR [RHU GM-CSF) AND RHU G-CSF ADMINISTRATION ON NEUTROPHIL CHEMILUMINESCENCE ASSAY IN PATIENTS FOLLOWING CYCLIC CHEMOTHERAPY/
M. Aslan et al., THE EFFECT OF RECOMBINANT HUMAN GRANULOCYTE MACROPHAGE-COLONY-STIMULATING FACTOR [RHU GM-CSF) AND RHU G-CSF ADMINISTRATION ON NEUTROPHIL CHEMILUMINESCENCE ASSAY IN PATIENTS FOLLOWING CYCLIC CHEMOTHERAPY/, Cancer immunology and immunotherapy, 47(3), 1998, pp. 176-181
Secondary infections related to neutropenia and functional defects of
phagocytes are common consequences in patients treated for cancer. The
hematopoietic colony-stimulating factors (CSF) have been introduced i
nto clinical practice as additional supportive measures that can reduc
e the incidence of infectious complications in patients with cancer an
d neutropenia. The aim of this study was to determine the role of gran
uolcyte/macrophage(GM)-CSF and granulocyte(G)-CSF in enhancing in vivo
human neutrophil function. A luminol-dependent chemiluminescence assa
y was developed to evaluate whether the repair in neutropenia accompan
ies the ability of neutrophils to function. A dose of 5 mu g G-CSF kg(
-1) day(-1) [recombinant human (rHu) G-CSF; filgrastim] or 250 mu g GM
-CSF m(-2) day(-1) (rHu GM-CSF; molgramostim) was administered subcuta
neously once daily to 12 metastatic cancer patients being treated with
different cytotoxic regimens. All injections of CSF were given after
the initiation of neutropenia and continued until the occurrence of an
absolute neutrophil recovery, rHu GMCSF and rHu G-CSF, administered o
nce daily at the 250 mu g m(-2) day(-1) and 5 mu g kg(-1) day(-1) leve
l, were effective in increasing the absolute neutrophil count and neut
rophil function, as measured by an automated chemiluminescence system.