FAST CHIRAL SEPARATION OF AMINO-ACID DERIVATIVES AND ACIDIC DRUGS BY CO-ELECTROOSMOTIC FLOW CAPILLARY-ELECTROPHORESIS WITH VANCOMYCIN AS CHIRAL SELECTOR

A fast chiral separation method for acidic enantiomers, including 9-fl uorenylmethyl chloroformate (FMOC) amino acid derivatives and ketoprof en, with vancomycin as chiral selector is presented. In this method, h exadimethrine bromide (HDB), a polycationic polymer, was added to the run buffer as electroosmotic flow (EOF) modifier. The reversed EOF mig rated in the same direction as the anionic analytes. Consequently, the separation time was shortened. Another advantage of using HDB as buff er additive is that the adsorption of vancomycin onto the capillary wa ll was minimized, hence, the separation efficiency was improved. The e ffects of buffer pH and vancomycin concentration on separation were in vestigated. Base line chiral separation of 12 FMOC-amino acid derivati ves, ketoprofen and drug intermediate 6,5'6'-bismethylenedioxybiphenyl -2,2'-dicarboxylic acid were obtained. The separation time for each en antiomers was not more than 4.5 min, and the average efficiency of 3.2 .10(5) plates/m was obtained. (C) 1998 Elsevier Science B.V. All right s reserved.