IDENTIFICATION OF SPECIFIC NUCLEAR-PROTEIN KINASE-C ISOZYMES AND ACCELERATED PROTEIN-KINASE C-DEPENDENT NUCLEAR-PROTEIN PHOSPHORYLATION DURING MYOCARDIAL-ISCHEMIA

Protein kinase C (PKC) has been suggested to mediate, at least: in par t, multiple processes in the pathophysiological sequelae of myocardial isehemia, The present study demonstrates that the epsilon, eta and io ta isozymes of PKC are translocated to nuclei in response to brief int ervals of global ischemia as well as reperfusion of ischemic rat myoca rdium. Concomitant with the translocation of PKC isozymes to nuclei du ring ischemia, increased PKC-mediated nuclear protein phosphorylation mas observed, Taken together, the present results demonstrate that nuc lear signaling mechanisms are activated during myocardial ischemia tha t include PKC translocation and PKC-mediated nuclear protein phosphory lation. (C) 1998 Federation of European Biochemical Societies.