LOCALIZATION OF HUC (ELAVL3) TO CHROMOSOME 19P13.2 BY FLUORESCENCE IN-SITU HYBRIDIZATION UTILIZING A NOVEL TYRAMIDE LABELING TECHNIQUE

HuC is a neural-specific member of the Elav family of RNA-binding prot eins. This highly conserved gene family plays a crucial role in neurog enesis, and HuC (HGMW-approved symbol ELAVL3) is expressed at an early stage of neural development. Using a novel tyramide fluorescence in s itu hybridization (T-FISH) technique, we localized HuC to chromosome 1 9p13.2. This localization was confirmed by radiation hybrid mapping an d coincides with that of HuR (HGMW-approved symbol ELAVL1), another el av family member. Dual T-FISH analysis with HuC and HuR probes, howeve r, indicated distinct loci, with HuC being centromeric to HuR. This st udy demonstrates the utility of T-FISH in colocalizing two genes on th e same chromosomal preparation using only biotinylated probes. (C) 199 8 Academic Press.