REGIONAL ASSIGNMENT AND EXPRESSION ANALYSIS OF 29 EXPRESSED SEQUENCE TAGS MAPPED TO CHROMOSOME-3

Of 311 expressed sequenced tags (ESTs) mapped to single human chromoso mes by analysis of a monochromosome somatic cell hybrid panel, 29 were localized to chromosome 3. Analysis of somatic cell hybrid lines cont aining different regions of chromosome 3 has enabled the regional assi gnment of these 29 ESTs to 13 of 23 intervals covering chromosome 3. N orthern analysis of 25 of the EST clones has provided information on t he pattern of expression of potential genes represented by these trans cripts in 16 human tissue types. Nine of the clones hybridized solely to a transcript(s) in the testis, 12 hybridized to transcripts in test is and other tissues, and 4 hybridized with transcripts in testis and other tissues but in addition have testis-specific transcript sizes. T hese ESTs will provide useful markers throughout chromosome 3 for the development of physical and transcription maps. In addition, they prov ide candidate genes for disease loci mapping to the intervals defined by the chromosome 3 deletion panel. (C) 1998 Academic Press.