A SEX-AVERAGED GENETIC-LINKAGE MAP IN COASTAL DOUGLAS-FIR (PSEUDOTSUGA-MENZIESII [MIRB.] FRANCO VAR MENZIESII) BASED ON RFLP AND RAPD MARKERS

We have constructed a sex-averaged genetic linkage map in coastal Doug las-fir (Pseudotsuga menziesii [Mirb.] France var 'menziesii') using a three-generation outcrossed pedigree and molecular markers. Our resea rch objectives are to learn about genome organization and to identify markers associated with adaptive traits. The map reported here is comp rised of 141 markers organized into 17 linkage groups and covers 1,062 centiMorgans (cM). Of the markers positioned on the map, 94 were deri ved from a Douglas-fir complimentary-DNA (cDNA) library that was const ructed from new-growth needle tissue. Other markers include 11 Douglas fir genomic-DNAs, 20 loblolly pine (Pinus taeda L.) cDNAs, 15 random amplified polymorphic DNAs (RAPDs) and a PCR-amplified phytochrome pro be. A high degree of variation was detected in each of the two parents of our mapping population, and many of the restriction fragment lengt h polymorphism (RFLP) and RAPD phenotypes were complex. Marker data we re analyzed for linkage using mapping software JOINMAP version 2.0.