PARTIAL DNA-SEQUENCING OF DOUGLAS-FIR CDNAS USED FOR RFLP MAPPING

DNA sequences from 87 Douglas-fir (Pseudotsuga menziesii [Mirb.] Franc e) cDNA RFLP probes were determined. Sequences were submitted to the G enBank dbEST database and searched for similarity against nucleotide a nd protein databases using the BLASTn and BLASTx programs. Twenty-one sequences (24%) were assigned putative functions; 18 of which were fro m plant species. Six sequences aligned with conifer genes, including g enes from Douglas-fir. Similarities among the 87 sequences were reveal ed by analyses with FASTA, suggesting either redundancy or isoforms of the same gene. Assignment of putative functions to anonymous cDNA map ped markers will increase the understanding of structural gene organiz ation of the Douglas-fir genome.