GENETIC-ANALYSIS OF RICE CMS-WA FERTILITY RESTORATION BASED ON QTL MAPPING

The inheritance of fertility restoration of rice cytoplasmic male ster ility of the wild abortive type was studied by means of QTL mapping. T he two segregating populations examined showed high frequencies of hig hly sterile and highly fertile progenies, but a low frequency of parti ally sterile and partially fertile progenies. The distributions sugges ted that fertility restoration was mainly controlled by major genes. B ased on a linkage map constructed with 57 RFLP and 61 AFLP markers on a B1F1 population, composite interval mapping (CIM) revealed that the fertility was restored by the additive effects of two restorer loci lo cated on chromosome 10. One QTL, tightly linked to RFLP marker C1361 i n the middle of the long arm of chromosome 10, explained 71.5% of the phenotypic variance. The second QTL was located between RFLP markers R 2309 and RG257 on the short arm and explained 27.3% of the phenotypic variance. Similar results were obtained using the simple interval mapp ing (SIM) methods.