IDENTIFICATION OF HUMAN PAPILLOMA-VIRUS DNA-SEQUENCE IN THE HYPERPLASTIC EPITHELIUM OF AN ORAL DENTURE FIBROMA

The human papilloma virus (HPV) associated with hyperplastic epitheliu m in an oral denture fibroma was examined by southern blot hybridizati on. Extracted DNA was hybridized with full length linear HPV type 2a, 6b, 11, 16, 18, 31 and 33 DNAs as a mixed probe only under low stringe nt conditions. The hybridized bands digested with Bam HI and Eco RT we re approximately 8.8 kbp and 15 kbp, respectively. Thus the lesional H PV DNA was different from HPV types used as probes and was probably in tegrated into host cell chromosomal DNA judging by the off-size high m olecular weight bands. Considering the contaminating mesenchymal regio n and uninfected epithelial cells as well as the evidently limited hom ology with probe HPV DNAs, the virus copy number in infected cells was poorly defined. In situ antigen staining signals were widely detected in the hyperplastic epithelial layer.