Purpose: To evaluate the shear bond strength of one-bottle dentin bond
ing agents (DBA's) (Prime & Bond 2.1, ART Experimental, Syntac Single
Component) on pressurized human dentin. Materials and Methods: Freshly
prepared dentin specimens of human teeth were perfused with horse ser
um which was diluted 1:5 in physiologic saline. Three different types
of surface treatment were evaluated on the freshly prepared dentin. Gr
oup 1: One of the three one-bottle DBA's was applied onto freshly prep
ared dentin together with a cylinder of composite luting resin and cur
ed. Group 2: A provisional cement (Freegenol, without eugenol, or Temp
Bond, containing eugenol) was applied first on the dentin surface for
24 h. Only then was one of the three the one-bottle DBA's added onto
the dentin together with a cylinder of composite luting resin after cl
eaning the dentin surface with pumice. Group 3: One of the three one-b
ottle DBA's was applied first on the dentin surface and light-cured. T
hen a provisional cement (Freegenol, without eugenol, or Temp Bond, co
ntaining eugenol) was added for 24 h. After cleaning with pumice, the
respective one-bottle DBA was applied for a second time onto the denti
n together with a cylinder of composite luting resin and light-cured.
As control for Group 1 (freshly prepared dentin), the two- or three-st
ep DBA's ART Bond and Syntac were used in a similar way. As control fo
r Group 2 (single application of the DBA's after contamination of the
dentin with a provisional cement) and Group 3 (dual application of the
DBA's with intermediate contamination of the dentin with a provisiona
l cement) the two-step DBA ART Bond was used. After 1500 thermal cycle
s with constant imitation of intrapulpal pressure, shear bond strength
s were measured. Resulting shear bond strength values were displayed b
y means of a box plot and they were analyzed statistically by Student'
s t-Test or one-way ANOVA. Results: Lowest and highest mean shear bond
strength values were 0.26+/-0.47 MPa (single use of ART Bond with pri
or application of Temp Bond) and 16.34+/-5.02 MPa (dual use of ART Bon
d with intermediate application of Temp Bond). With respect to the sur
face treatment significant differences between the DBA's could be foun
d in all groups.