BIODEGRADATION OF CRUDE-OIL BY SOIL-MICROORGANISMS IN THE TROPIC

Five microorganisms, three bacteria and two yeasts, capable of degradi ng Tapis light crude oil were isolated from oil-contaminated soil in B angkok, Thailand. Soil enrichment culture was done by inoculating the soil in mineral salt medium with 0.5% v/v Tapis crude oil as the sole carbon source. Crude oil biodegradation was measured by gas chromatogr aphy method. Five strains of pure microorganisms with petroleum degrad ing ability were isolated: three were bacteria and the other two were yeasts. Candida tropicalis strains 7Y and 15Y were identified as effic ient oil degraders. Strain 15Y was more efficient, it was able to redu ce 87.3% of the total petroleum or 99.6% of n-alkanes within the 7-day incubation period at room temperature of 25 +/- 2 degrees C.