COPROTHERMOBACTER-PLATENSIS SP. NOV., A NEW ANAEROBIC PROTEOLYTIC THERMOPHILIC BACTERIUM ISOLATED FROM AN ANAEROBIC MESOPHILIC SLUDGE

Citation
C. Etchebehere et al., COPROTHERMOBACTER-PLATENSIS SP. NOV., A NEW ANAEROBIC PROTEOLYTIC THERMOPHILIC BACTERIUM ISOLATED FROM AN ANAEROBIC MESOPHILIC SLUDGE, International journal of systematic bacteriology, 48, 1998, pp. 1297-1304
Citations number
34
Categorie Soggetti
Microbiology
ISSN journal
00207713
Volume
48
Year of publication
1998
Part
4
Pages
1297 - 1304
Database
ISI
SICI code
0020-7713(1998)48:<1297:CSNANA>2.0.ZU;2-1
Abstract
A new anaerobic, proteolytic, moderately thermophilic bacterium, strai n 3R(T), was isolated from a methanogenic mesophilic reactor treating protein-rich wastewater. The cells were Gram-negative, non-spore-formi ng, non-motile rods. The DNA base composition was 43 mol% G+C. The opt imum ph and temperature for growth were 7.0 and 55 degrees C respectiv ely. The bacterium fermented gelatin, casein, bovine albumin, peptone and yeast extract. Glucose, fructose, sucrose, maltose and starch were poorly fermented. The major fermentation products from glucose were a cetate, CO2 and H-2 and, from gelatin, propionate was also detected. G rowth on glucose was stimulated by thiosulfate, which was reduced to s ulfide. Sulfate and nitrate were not reduced. 16S rRNA gene analysis r evealed that the isolated bacterial strain was phylogenetically relate d to Coprothermobacter proteolyticus (96.3 % sequence similarity), the only known species within the genus. DNA-DNA hybridization analysis d emonstrated a very low level of homology, indicating that the isolated strain and C. proteolyticus were not related at species level. Theref ore, it is proposed to classify the described strain in the genus Copr othermobacter as a new species, Coprothermobacter platensis. The type strain of C. platensis is strain 3R(T) (= DSM 11748(T)).