AN EXTENDED GENETIC-MAP OF RYE (SECALE-CEREALE L)

A genetic linkage map of rye consisting of 92 markers was constructed by using isozyme and molecular marker techniques. For this purpose an F-2 population of 137 individuals was established on which RFLP studie s with homologous and heterologous probes were performed. After establ ishing a reliable polymerase chain reaction (PCR) protocol, 280 random primers were screened for polymorphisms and 17 random amplified polym orphic DNA (RAPD) loci were mapped. The digestion of the template DNA prior to PCR increased the degree of polymorphism. Previously publishe d markers could also be integrated into this map by using the JoinMap computer program. The resulting linkage map comprises a total of 127 m arkers and spans a distance of about 760 cM.