BIOFILM SUSCEPTIBILITY TO BACTERIOPHAGE ATTACK - THE ROLE OF PHAGE-BORNE POLYSACCHARIDE DEPOLYMERASE

Biofilm bacteria Enterobacter agglomerans 53b and Serratia marcescens Serr were isolated from a food processing factory. A bacteriophage (SF 153b), which could infect and lyse strain 53b, was isolated from sewag e. This has been shown to possess a polysaccharide depolymerase enzyme specific for the exopolysaccharide (EPS) of strain 53b. Using batch c ulture and chemostat-linked Modified Robbins Device systems it was obs erved that SF153b could degrade the EPS of a mono-species biofilm (str ain 53b) and infect the cells. The disruption of the biofilm by phage was a combination of EPS degradation by the depolymerase and infection and subsequent cell lysis by the phage. Strain Serr biofilms were not susceptible to the phage and the biofilm EPS was not degraded by the phage glycanase, with the result that the biofilm was unaffected by th e addition of SF153b phage. Scanning electron microscopy confirmed tha t specific phage could extensively degrade susceptible biofilms and co ntinue to infect biofilm bacteria whilst EPS degradation was occurring .