DNA-PLOIDY DETERMINATION OF EARLY MOLAR PREGNANCIES BY IMAGE-ANALYSIS- COMPARISON TO HISTOLOGIC CLASSIFICATION

Citation
A. Gschwendtner et al., DNA-PLOIDY DETERMINATION OF EARLY MOLAR PREGNANCIES BY IMAGE-ANALYSIS- COMPARISON TO HISTOLOGIC CLASSIFICATION, Archives of pathology and laboratory medicine, 122(11), 1998, pp. 1000-1004
Citations number
22
Categorie Soggetti
Pathology,"Medical Laboratory Technology","Medicine, Research & Experimental
Journal title
Archives of pathology and laboratory medicine
ISSN journal
00039985 → ACNP
Volume
122
Issue
11
Year of publication
1998
Pages
1000 - 1004
Database
ISI
SICI code
0003-9985(1998)122:11<1000:DDOEMP>2.0.ZU;2-L
Abstract
Background.-To improve histologic diagnosis of molar pregnancies, upda ted guidelines have been proposed recently. These guidelines take into account that less developed molar gestations differ from their fully developed counterparts. Objective.-To test the validity of these crite ria by correlating histologic diagnosis with ploidy determination acco mplished by means of image analysis. Design.-Fifty archival cases of e arly molar pregnancy were reclassified according to the new criteria. The diagnosis had to be changed from partial to complete hydatidiform mole (PM to CM, respectively) in 9 cases and from CM to PM in 4 cases. DNA image cytometry could be performed in 40 cases (CM, n = 21; PM, n = 99). Results.-There was 100% agreement between histologic diagnosis and a diploid or polyploid histogram in CM and 79% agreement between triploidy and PM, when the updated diagnostic criteria were used. This represents an improvement compared with diagnoses made with former cr iteria. Nevertheless, problems of correct classification remain: In 3 cases classified as PMs, fetal remnants were accompanied by the histol ogic appearance of a CM, These 3 cases could represent either a true e mbryonic development in CM or a twin gestation with one normal pregnan cy and one mole, or they could belong to a (very rare) third type of m ole. All of them show the same risk of persistent trophoblastic: disea se observed in classic CM. Conclusions.-As the groups at risk for deve loping persistent trophoblastic disease can be identified by their DNA histograms, ploidy analysis would be desirable in addition to histolo gic examination.