MIB-1 AND PC-10 IMMUNOSTAINING FOR THE ASSESSMENT OF PROLIFERATIVE ACTIVITY IN PRIMARY ACQUIRED MELANOSIS WITHOUT AND WITH ATYPIA

Aims-To compare the proliferative activity of intraepithelial melanocy tes in primary acquired melanosis (PAM) without atypia and PAM with at ypia by immunohistochemical staining for the Ki-67 antigen and the pro liferating cell nuclear antigen (PCNA). Methods-Formalin fixed, paraff in embedded sections from 35 archival specimens of PAM without atypia (n=19) and with atypia (n=16) were studied by immunostaining with MIB- 1 and PC-10 monoclonal antibodies that react with the Ki-67 antigen an d PCNA respectively. The results were calculated as the mean number of positive cells per eyepiece grid. All specimens were evaluated by two masked observers, and the interobserver reproducibility was assessed. Results-The means of the positive cell count in PAM with atypia were significantly higher compared with PAM without atypia for both observe rs, in both the PC-10 and the MIB-1 stained sections. In a linear leas t square model that estimated the interobserver and between group vari ation, the difference of MIB-1 and PC-10 positive cell count between P AM without and with atypia remained highly significant. The difference between the observers was not significant. Conclusions-Immunostaining with MIB-1 and PC-10 demonstrated that PAM with atypia has higher pro liferative activity than PAM without atypia. This method was found to be reproducible between different observers.