MONOAMINES, AMINO-ACIDS AND ACETYLCHOLINE IN THE PREOPTIC AREA AND ANTERIOR HYPOTHALAMUS OF RATS - MEASUREMENTS OF TISSUE-EXTRACTS AND IN-VIVO MICRODIALYSATES
M. Yasumatsu et al., MONOAMINES, AMINO-ACIDS AND ACETYLCHOLINE IN THE PREOPTIC AREA AND ANTERIOR HYPOTHALAMUS OF RATS - MEASUREMENTS OF TISSUE-EXTRACTS AND IN-VIVO MICRODIALYSATES, Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 121(1), 1998, pp. 13-23
A microbore column high-performance liquid chromatography (HPLC) syste
m was used to measure neurotransmitters in tissue extracts and in vivo
microdialysates obtained from the preoptic area (PO) and anterior hyp
othalamus (AH) of rats. The extracts contained norepinephrine, epineph
rine, 3,4-dihydroxyphenylacetic acid (DOPAC), dopamine, 5-hydroxyindol
eacetic acid (5-HIAA), homovanillic acid (HVA), 5-hydroxytryptamine (5
-HT), aspartate, glutamate, GABA, acetylcholine (ACh) and choline. The
microdialysates obtained from the PO and AH of freely moving rats con
tained all of these substances except for norepinephrine, epinephrine,
dopamine, and 5-HT. During collection of microdialysate from the PO a
nd AH, core body temperature and locomotor activity were simultaneousl
y measured by means of telemetry. The locomotor activity and body temp
erature increased during the night. This was accompanied by increased
levels of 5-HIAA. The results suggest that serotonergic neuronal mecha
nisms in the PO and AH may be involved in hypothalamic regulation of s
pontaneous behaviors and body temperature. (C) 1998 Elsevier Science I
nc. All rights reserved.