EVALUATION OF RESINS FOR ON-BEAD SCREENING - A STUDY OF PAPAIN AND CHYMOTRYPSIN SPECIFICITY USING PEGA-BOUND COMBINATORIAL PEPTIDE LIBRARIES

TentaGel, ArgoGel and PEGA resins were evaluated for on-bead biologica l screening, using a fluorescently-labelled peptide attached to each a nd assayed for papain activity. Peptide attached to PEGA was cleaved i n near quantitative yield at the expected sites, whilst an identical s equence on TentaCel and ArgoGel beads was hydrolysed in very low yield s and nonspecifically on ArgoGel. The compatibility of PEGA with enzym es was further demonstrated by the determination of subsite specificit ies of papain and chymotrypsin using PEGA-bound peptide libraries, whi ch proved to be similar to those observed in free solution. (C) 1998 E lsevier Science Ltd. All rights reserved.