TRIMETAZIDINE REVERSES DELETERIOUS EFFECTS OF ISCHEMIA-REPERFUSION INTHE ISOLATED-PERFUSED PIG-KIDNEY MODEL

Background: Delayed graft function has remained an important complicat ion after renal transplantation. Methods: The purpose of this study wa s to evaluate Euro-Collins (EC) plus trimetazidine (TMZ) in comparison with standard EC solution after 24- or 48-hour cold storage. The norm othermic isolated perfused pig kidney technique combined with proton n uclear magnetic spectroscopy was used. Results: The study verified tha t TMZ plus EC had a beneficial preservation effect over EC in terms of better perfusate flow rate at both 24 and 48 h (p < 0.01 and p < 0.00 1, respectively). In addition, TMZ also was beneficial in terms of inc reased glomerular filtration rate, better proximal tubular functions, and less tubular injury markers. Lipid peroxidation, evaluated by malo ndialdehyde renal tissue levels, was decreased in kidney homogenates p reserved with TMZ, particularly after 48-hour cold storage. Citrate ex cretion which reflects a better intracellular pH regulation was detect ed in urine from kidneys preserved with TMZ. Histological data paralle led findings of the above when comparing cellular injury factors such as vacuolization, necrosis, tubular structure, and interstitial edema. Conclusion: These results indicate that, under the conditions of our experiments, the addition of TMZ to EC solution increased the preserva tion quality of kidneys particularly after prolonged cold ischemia.