APPLICATION OF HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUORESCENCE DETECTION FOR DETERMINATION OF NITROARENES, AFTER THEIR REDUCTION TO AROMATIC-AMINES

Reversed-phase high performance liquid chromatography with fluorescenc e detection has been used for qualitative/quantitative determination o f nitroarenes after their reduction to corresponding aromatic amines. Two methods of nitroarenes reduction to aromatic amines were compared. The reduction efficiency of nitroarene standards using NaHS was found to be higher than NaBH4/CuCl2 reduction system. 2-Nitrofluorene and 1 -nitropyrene were identified in the organic extract of dusts collected in Upper Silesia Region with detection limits of 9 pg and 1pg respect ively. It has been shown that the contents of nitroarenes were within the range of 4.3-5.0 mu g g(-1) of dust.