Saturating the region of the polycystic kidney disease gene with NotI linking clones.

Authors
Himmelbauer, Heinz Germino, Greg G. Ceccherini, Isabella Romeo, Giovanni Reeders, Stephen T. Frischauf, Anna-Maria
Citation
Himmelbauer, Heinz et al., Saturating the region of the polycystic kidney disease gene with NotI linking clones., American journal of human genetics , 48-I(2), 1991, pp. 325-334
Journal title
American journal of human genetics → ACNP
ISSN journal
00029297
Volume
48-I
Issue
2
Year of publication
1991
Pages
325 - 334
Database
ACNP
SICI code
Abstract
A NotI-linking library was constructed from a radiation hybrid containing fragments of human chromosome 16.The clones were mapped on a panel of somatic cell hybrids, and 10 different NotI site-containing clones were localized close to and between genetic markers flanking the PKD1 locus.With pulsed-field gel analysis the clones were shown to be distributed over four adjacent ClaI fragments covering 1,200 kb.