TYPE-1 CU STRUCTURE OF BLUE NITRITE REDUCTASE FROM ALCALIGENES-XYLOSOXIDANS GIFU-1051 AT 2.05 ANGSTROM RESOLUTION - COMPARISON OF BLUE AND GREEN NITRITE REDUCTASES

Authors
INOUE T GOTOWDA M",DELIGEER,"KATAOKA K YAMAGUCHI K SUZUKI S WATANABE H GOHOW M KAI Y
Citation
T. Inoue et al., TYPE-1 CU STRUCTURE OF BLUE NITRITE REDUCTASE FROM ALCALIGENES-XYLOSOXIDANS GIFU-1051 AT 2.05 ANGSTROM RESOLUTION - COMPARISON OF BLUE AND GREEN NITRITE REDUCTASES, Journal of Biochemistry (Tokyo), 124(5), 1998, pp. 876-879
Citations number
31
Categorie Soggetti
Biology
Journal title
Journal of Biochemistry (Tokyo) → ACNP
ISSN journal
0021924X
Volume
124
Issue
5
Year of publication
1998
Pages
876 - 879
Database
ISI
SICI code
0021-924X(1998)124:5<876:TCSOBN>2.0.ZU;2-6
Abstract
The crystal structure of the blue nitrite reductase from Alcaligenes x ylosoxidans GIFU 1051 (AxgNIR) has been determined at 2.05 Angstrom re solution, AxgNIR contains both type 1 and 2 Cu sites, the geometry of the former being distorted tetrahedral. The superpositioning of the ty pe I Cu sites in the blue enzyme and a green nitrite reductase reveale d that the orientation of the Met150 side chain differed. The deviatio n of the S-delta(Met150) atom from the axial position of the NNS plane formed by two N-delta(His95 and His145) and one S-beta(Cys136) atom c aused the difference in the colors of the enzymes, Le. blue and green.