EFFECT OF PROTEIN-KINASE INHIBITORS ON PRIMARY ANTIBODY INDUCTION IN TISSUE-CULTURES

The effects of protein-kinase-inhibitors (PKIs) on protein kinase C (P KCs) i.e., staurosporin, calphostin C, H-7, H-8, H-9, on phosphatidyl inositol 3-proteinkinase (PI3-K) i.e., wortmannin, and on protein tyro sine kinase (PTKs) i.e., genistein; herbimycin A, sanguinarin, lavendu stin 4 and B were tested:on the induction phase of the primary Ab-resp onse in vitro. The inhibitory action of PKIs was the highest with herb imycin A, sanguinarin, H-9 and wortmannin. Although wortmannin inhibit s the function of T-lymphocytes (Taub et al., 1997, Shi et al., 1997), we believe that this communication is the first report of PKIs immuno suppressive action on the inductive steps of Ab-formation. (C) 1998 Pu blished by Elsevier Science Ltd.