NEURONAL PATTERNING BY BMPS - A REQUIREMENT FOR GDF7 IN THE GENERATION OF A DISCRETE CLASS OF COMMISSURAL INTERNEURONS IN THE MOUSE SPINAL-CORD

Inductive factors are known to direct the regional differentiation of the vertebrate central nervous system (CNS) but their role in the spec ification of individual neuronal cell types is less clear. We have exa mined the function of GDF7, a BMP family member expressed selectively by roof plate cells, in the generation of neuronal cell types in the d orsal spinal cord. We find that GDF7 can promote the differentiation i n vitro of two dorsal sensory interneuron classes, D1A and D1B neurons . In Gdf7-null mutant embryos, the generation of D1A neurons is elimin ated but D1B neurons and other identified dorsal interneurons are unaf fected. These findings show that GDF7 is an inductive signal from the roof plate required for the specification of neuronal identity in the dorsal spinal cord and that GDF7 and other BMP family members expresse d by the roof plate have non-redundant functions in vivo. More general ly, these results suggest that BMP signaling may have a prominent role in the assignment of neuronal identity within the mammalian CNS.