AN INTEGRATED TRANSCRIPT MAP OF HUMAN-CHROMOSOME 1P35-P36

The distal short arm of human chromosome 1 (Ip) is rearranged in a var iety of malignancies, and several genetic diseases also map to this re gion. We have constructed an integrated transcript map to precisely de fine the positions of genes and expressed sequence tags (ESTs) previou sly mapped to 1p35-p36, a region spanning approximately 40 Mb. To anch or the integrated map, a framework genetic map was constructed with 24 genetic markers and a marker order of >1000:1 odds, yielding an avera ge resolution of 2.8 cM. An additional 106 genetic markers were locali zed relative to the framework genetic map. To place markers more preci sely within 1p35-p36, a chromosome 1-specific, radiation-reduced hybri d (RH) panel was created. Individual DNA fragments of the RH panel wer e identified and ordered by PCR with the framework. genetic map. A tot al of 250 markers, including 142 genes and ESTs, were mapped by PCR ag ainst the RH panel. The map has an observed resolution of 800 kb, and the results closely match and more precisely define previous mapping i nformation for most markers. This map will help to identify candidate genes for genetic diseases mapping to distal Ip and is fully integrate d with existing genetic and RH maps of the human genome. (C) 1997 Acad emic Press.